The Fact About high performance liquid chromatography That No One Is Suggesting
The Fact About high performance liquid chromatography That No One Is Suggesting
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物質の持つ特定波長の光を吸収する性質を利用した検出器。次のようなものが存在している。
内部にカラムを収納して加熱あるいは冷却を行い、カラムの温度を制御する装置。カラムヒーターとも称する。
Acid–base chemistry isn't the only illustration of a secondary equilibrium response. Other examples consist of ion-pairing, complexation, and the interaction of solutes with micelles. We'll look at the very last of these in Chapter 12.7 after we focus on micellar electrokinetic capillary chromatography.
Bubbling an inert gasoline throughout the mobile period releases unstable dissolved gases. This process is referred to as sparging.
). If the detector is really a diode array spectrometer, then we can also Screen the result as A 3-dimensional chromatogram that demonstrates absorbance being a function of wavelength and elution time.
. During the load situation a sample loop—which is accessible in a number of dimensions starting from 0.5 μL to five mL—is isolated from your cell phase and open up into the ambiance. The sample loop is stuffed utilizing a syringe with a capability a number of occasions that with the sample loop, with excess sample exiting with the squander line.
규제 약물(마약, 합성 마약, 대마, 각성제, 향정신성 의약품, 아편양제제 등), 반도핑 관련(금지 물질, 금지 약물, 스테로이드 등), 약물 대사물
It achieves this by exploiting the differing how HPLC works interactions of sample compounds with two critical phases: the cellular section and also the stationary period. Knowledge the core components of the HPLC system and their roles is important for profitable Examination.
Ghost peaks are extraneous peaks that seem in the chromatogram but Will not correspond to any components during the sample. These can complicate knowledge Examination. Here are a few likely triggers and answers:
The a few red circles are binary mobile phases developed by combining equivalent volumes of your pure cell phases. The ternary mobile phase proven with the purple circle is made up of all 3 on the pure mobile phases.
The column is the separation chamber exactly where the magic of HPLC happens. It properties the stationary phase, a packed bed of microscopic particles.
Prevalent mobile stage modifiers like acids and bases is usually extra to fine-tune the conversation amongst analytes and also the column. These modifiers can:
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